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Purification of Vero cell derived live replication deficient influenza A and B virus by ion exchange monolith chromatography

✍ Scribed by Banjac, Marko; Roethl, Elisabeth; Gelhart, Franz; Kramberger, Petra; Jarc, Barbara Lah; Jarc, Marko; Štrancar, Aleš; Muster, Thomas; Peterka, Matjaž

Book ID: 121708508
Publisher: Elsevier Science
Year: 2014
Tongue: English
Weight: 540 KB
Volume: 32
Category: Article
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2014.02.086

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## Abstract A process comprising of size‐exclusion chromatography (SEC) and anion‐exchange chromatography (AEC) was investigated for downstream processing of cell culture‐derived influenza A virus. Human influenza virus A/PR/8/34 (H1N1) was propagated in serum‐free medium using MDCK cells as a host