Purification of tumor-specific antigens. An overview of the relevance to human colon carcinoma

✍ Scribed by Dr. Barry D. Kahan; Baldwin H. Tom; Margalit B. Mokyr; Lynne P. Rutzky; Neal R. Pellis

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 457 KB
• Volume: 36
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(197512)36:6<2449::aid-cncr2820360629>3.0.co;2-w

✦ Synopsis

Methods which dissociate intramolecular noncovalent bonds have been used to prepare soluble derivatives of cell-surface antigens. Applications of these techniques to human colon carcinoma are underway. Continuous tissue-culture strains derived from primary lesions were developed and shown to be composed of malignant epithelial elements. Parallel data on the preparation and activity of soluble materials in a murine model methylcholanthrene system reveal that although cultured cells are a satisfactory source for antigen extraction, they are poor targets of the immune response. The development of methods to quantitate the biologic activity of colon-specific, soluble materials may provide indicator systems to define the antigenic determinants, to permit purification, and to serve as assays of the efficacy of immunotherapy.