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Purification of transfection-grade plasmid DNA from bacterial cells with superparamagnetic nanoparticles

✍ Scribed by Chen-Li Chiang; Ching-Shan Sung


Book ID
113724086
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
344 KB
Volume
302
Category
Article
ISSN
0304-8853

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## Abstract We have shown previously that a sequence‐specific DNA‐binding protein based on the Lac repressor protein can isolate pre‐purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically