Affinity purification of plasmid DNA dir
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Richard A.J. Darby; Gareth M. Forde; Nigel K.H. Slater; Anna V. Hine
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Article
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2007
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John Wiley and Sons
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English
⚖ 182 KB
## Abstract We have shown previously that a sequence‐specific DNA‐binding protein based on the Lac repressor protein can isolate pre‐purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials were disappointing with impractically