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Purification of the glycoprotein allergen Ag7 from mugwort pollen by concanavalin A affinity chromatography

โœ Scribed by Nilsen, Bente Merete; Smestad Paulsen, Berit; Clonis, Yannis; Mellbye, Kari Svane

Book ID
122573264
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
875 KB
Volume
16
Category
Article
ISSN
0168-1656

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โœ Michael G. Brattain; Michael E. Marks; Thomas G. Pretlow II ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 426 KB

This report describes the use of affinity chromatography on Sepharose-bound concanavalin A for the purification of horseradish peroxidase. Samples of horseradish peroxidase with A .,03:A280 ratios ranging from 0.62 to 2.45 were purified to AIOa:AZSO ratios ranging from approximately 2.8 to 3.1 with