Purification of staphylococcal enterotoxin E

✍ Scribed by Dr. Rossalyn D. Brehm; Howard S. Tranter

• Book ID: 102551886
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 435 KB
• Volume: 1
• Category: Article
• ISSN: 1056-9014
• DOI: 10.1002/nt.2620010409

✦ Synopsis

Staphylococcal enterotoxin E has been purified by a combination of Red-A ligand chromatography and Mono-S (cationexchange) chromatography. Commencing with 30 tities of fermenter-grown culture the method has been used to purify 17 mg of toxin, giving a purification yield of around 40%. The purity of the toxin was over 98% when analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, but demonstrated three isoelectric forms by isoelectric focusing, with pl values of 7.6,7.4, and 7.0. This method adds to the versatility of the Red-A matrix system which can also be used for the purification of staphylococcal enterotoxins A, B, and Cp.