Purification of Schwann cells from adult rats by differential detachment
β Scribed by Wei Wu; Yu-Qing Jin; James D. Kretlow; Liang Xu; Hui-Chuan Duan; Zuo-Liang Qi
- Book ID
- 106364477
- Publisher
- Springer Netherlands
- Year
- 2009
- Tongue
- English
- Weight
- 304 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0141-5492
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To elucidate the molecular mechanisms underlying the development of diabetic neuropathy, we isolated the Schwann cells from the sciatic nerves of adult rats and characterized the polyol pathway activity. Despite the presence of aldose reductase (AR) activity, no accumulation of sorbitol was observed
## Abstract We present here a fast protocol that could be used to obtain highly purified cultures of maximal proliferating adult rat Schwann cells. These adult rat Schwann cells can be transfected in a nonbiological way using the physical transfection method of electroporation. Schwann cells are de