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Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme

โœ Scribed by Brian Cass; Phuong Lan Pham; Amine Kamen; Yves Durocher

Book ID
116828001
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
632 KB
Volume
40
Category
Article
ISSN
1046-5928

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## Abstract An integrated product recovery system was developed to separate urokinase from the cell culture broth of human kidney cells HT1080. Supermacroporous monolithic cryogels provided ideal matrices with respect to surface and flow properties for use as cell culture scaffold as well as for af