Purification of recombinant histidine-tag streptolysin O using immobilized metal affinity expanded bed adsorption (IMA-EBA)

Facilitated downstream processing of a h

Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded-bed adsorption

✍ R. H. Clemmitt; H. A. Chase 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 202 KB 👁 2 views

The facilitated downstream processing of an intracellular, polyhistidine-tagged protein, glutathione Stransferase [GST-(His) 6 ], direct from unclarified E. coli homogenates using expanded beds of STREAMLINE chelating, has been investigated. A series of pilot experiments were used to develop prepara