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Purification of purkinje cells from postnatal cerebellum by cell sorting

โœ Scribed by Mineko Tomomura; Michisuke Yuzaki

Book ID
117364797
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
113 KB
Volume
31
Category
Article
ISSN
0168-0102

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## Abstract Trypsinized cells of newborn mouse cerebellum have been separated by velocity sedimentation at unit gravity in shallow gradients of Ficoll. The two main technical difficulties were formation of gels around the dissociated cells and clumping of cells before and during the sedimentation p