Purification of Pseudomonas cytochrome oxidase (or nitrite reductase) by immunological methods

✍ Scribed by Maria Chiara Silvestrini; Gennaro Citro; Alfredo Colosimo; Alberto Chersi; Romano Zito; Maurizio Brunori

• Book ID: 102984829
• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 571 KB
• Volume: 129
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90556-0

✦ Synopsis

A new purification procedure for the cytochrome oxidase from Pseudomonas aeruginosa based on immunoaffinity chromatography has been compared with the biochemical method and shown to be (i) fully competitive in terms of chemical homogeneity and enzymatic properties of the purified protein (ii) slightly less efficient in terms of total recovery and (iii) much more convenient in terms of the time required. A further evolution of the method that minimizes the number of purification steps and any stress to the native structure of the protein is suggested.