Partial purification of microsomal signa
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R. Keith Baker; Gian Paolo Bentivoglio; Mark O. Lively
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Article
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1986
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John Wiley and Sons
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English
β 532 KB
Signal peptidase has been purified approximately 600-fold from hen oviduct microsomes. Treatment of microsomes with ice-cold sodium carbonate at pH 11.5 removes soluble and extrinsic membrane proteins prior to solubilization of signal peptidase with Nonidet P-40. After dialysis to pH 8.2, the solubi