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Purification of protein phosphatase from hen oviduct

✍ Scribed by Keiko Kanayama; Kenji Wada; Akira Negami; Hirohei Yamamura; Tadashi Tanabe


Book ID
115916949
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
309 KB
Volume
184
Category
Article
ISSN
0014-5793

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Partial purification of microsomal signa
✍ R. Keith Baker; Gian Paolo Bentivoglio; Mark O. Lively πŸ“‚ Article πŸ“… 1986 πŸ› John Wiley and Sons 🌐 English βš– 532 KB

Signal peptidase has been purified approximately 600-fold from hen oviduct microsomes. Treatment of microsomes with ice-cold sodium carbonate at pH 11.5 removes soluble and extrinsic membrane proteins prior to solubilization of signal peptidase with Nonidet P-40. After dialysis to pH 8.2, the solubi