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Purification of plasmid DNA using a multicompartment electrolyser separated by ultrafilter membranes

✍ Scribed by Zhijun Wang; Guowei Le; Yonghui Shi; Grzegorz Węgrzyn


Book ID
110324699
Publisher
Springer Netherlands
Year
2002
Tongue
English
Weight
79 KB
Volume
24
Category
Article
ISSN
0141-5492

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Large-scale purification of plasmid DNA
✍ Gyan Chandra; Pankaj Patel; Thomas A. Kost; John G. Gray 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 837 KB

The large-scale purification of plasmid DNA was achieved using fast protein liquid chromatography on a Hi-Load Q Sepharose column. This method allows for the purification of plasmids starting from crude plasmid DNA, prepared by a simple alkaline lysis procedure, to pure DNA in less than 5 h. In cont