Large-scale purification of plasmid DNA
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Gyan Chandra; Pankaj Patel; Thomas A. Kost; John G. Gray
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Article
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1992
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Elsevier Science
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English
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The large-scale purification of plasmid DNA was achieved using fast protein liquid chromatography on a Hi-Load Q Sepharose column. This method allows for the purification of plasmids starting from crude plasmid DNA, prepared by a simple alkaline lysis procedure, to pure DNA in less than 5 h. In cont