A simple, scalable method for purification of plasmid DNA is described. The method includes modification of the classical alkaline-lysis-based plasmid extraction method by extending the solubilization step from less than 30 min to 24 h. The extraction is followed by the novel use of tangential flow
Purification of plasmid by high-performance gel filtration
β Scribed by Yamaseki, Y. ;Kato, Y. ;Murotsu, T. ;Fukushige, S. ;Matsubara, K.
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 166 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0935-6304
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