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Purification of placental alkaline phosphatase

โœ Scribed by Z. Ahmed; E.J. King


Book ID
115789331
Publisher
Elsevier Science
Year
1960
Weight
646 KB
Volume
40
Category
Article
ISSN
0006-3002

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๐Ÿ“œ SIMILAR VOLUMES


Purification and partial characterizatio
โœ Per ร…ke Holmgren; Torgny Stigbrand; Gunhild Beckman ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Springer ๐ŸŒ English โš– 461 KB

The I variant of placental alkaline phosphatase was purified to homogeneity by means of DEAE-cellulose chromatography, isoelectric focusing, and gel filtration on AcA-34. The specific activity of the I variant was found to be 3.33 micronkat/mg. The enzyme is a dimer with an isoelectric point of 4.6

Purification and partial characterizatio
โœ Per ร…ke Holmgren; Torgny Stigbrand ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Springer ๐ŸŒ English โš– 630 KB

The two most common variants of placental alkaline phosphatase, the F and S variants, were purified to homogeneity and characterized. Their molecular weights were determined by equilibrium ultracentrifugation and sodium dodecylsufate polyacrylamide gel electrophoresis, which gave almost identical va