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Purification of Oligonucleotides by High Affinity, Low Molecular Weight Displacers

✍ Scribed by Abhinav A. Shukla; Ranjit R. Deshmukh; J. A. Moore; Steven M. Cramer

Book ID
109386150
Publisher
American Institute of Chemical Engineers
Year
2000
Tongue
English
Weight
117 KB
Volume
16
Category
Article
ISSN
8756-7938

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Human low-molecular-weight kininogen (LK) was shown by fluorescence titration to bind two molecules of cathepsins L and S and papain with high affinity. By contrast, binding of a second molecule of cathepsin H was much weaker. The 2:1 binding stoichiometry was confirmed by titration monitored by los