Purification of nucleosidyl peptides by chromatography on dihydroxyboryl-substituted polyacrylamide and cellulose

✍ Scribed by Anjanayaki E. Annamalai; Pranab K. Pal; Roberta F. Colman

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 602 KB
• Volume: 99
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90046-0

✦ Synopsis

A new column chromatographic method is presented for the purification of peptides which are covalently bound to nucleoside analogs (nucleosidyl peptides). The procedure involves complex formation between the cis-diol moiety of the nucleosidyl peptide and the dihydroxyborylphenyl group which is linked either to polyacrylamide or to cellulose as a support; thus, the nucleosidyl peptides can be reversibly bound to the column while all other peptides are eluted in the void volume. This approach is exemplified by the purification of two peptides of rabbit muscle pyruvate kinase labeled with 5'-p-fluorosulfonylbenzoyl adenosine and one peptide of bovine liver glutamate dehydrogenase modified with 5'-p-fluorosulfonylbenzoyl guanosine. The method may be generally applicable to the purification of peptides resulting from the affinity labeling of nucleotide sites in proteins.