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Purification of nucleoside triphosphatases from pea seedling ribosomes

✍ Scribed by S. Matsushita; I.D. Raacke


Book ID
115743244
Publisher
Elsevier Science
Year
1968
Weight
241 KB
Volume
166
Category
Article
ISSN
0005-2787

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A chromatographic procedure was developed for the purification of ribosomes. Young pea plants were ground in a solvent containing polyethylene glycol, magnesium acetate, sucrose, and tris(l~ydroxymetl~yl)aminomethane-acetic acid buffer (pH 7). The slurry was poured onto a column of cellulose and san