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Purification of neutral protease by dye affinity chromatography

✍ Scribed by Nancy B. Iannucci; Agustín A. Navarro del Cañizo; Osvaldo Cascone

Book ID
111634226
Publisher
Springer-Verlag
Year
2003
Tongue
English
Weight
102 KB
Volume
104
Category
Article
ISSN
0273-2289

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Purification of human erythrocyte phosphoglycerate kinase by dye ligand affinity chromatography
✍ Josiane Chen-Marotel; Yves Blouquit; Raymonde Rosa; Marie-Claude Calvin 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 906 KB

A new method for the purification of human erythrocyte phosphoglyceratekinase involving affinity chromatography on dye-ligand media (Red A), in the presence of 3-phosphoglycerate and ATP, is described. The method is rapid and technically simple. The purity of the enzyme was verified by electrophores