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Purification of Monoclonal Antibodies from Cell-Culture Supernatant by Use of Anion-Exchange Convective Interaction Media (CIM) Monolithic Columns

โœ Scribed by Ambur P. Dhivya; Bellie Prem Kumar; Rajasekar R. Prasanna; Naatamai S. Jayaprakash; Mookambeswaran A. Vijayalakshmi


Book ID
111792197
Publisher
Springer
Year
2010
Tongue
English
Weight
449 KB
Volume
72
Category
Article
ISSN
0009-5893

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A shortcut purification sequence for therapeutic proteins should consist of three steps: capture, purification, and polishing. Special emphasis has been put on direct capture of human monoclonal antibodies from culture supernatants with ion-exchangers avoiding pretreatment steps such as desalting, d