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Purification of lipases, phospholipases and kinases by heparin-Sepharose chromatography

✍ Scribed by Akhlaq A. Farooqui; Hsiu-Chiung Yang; Lloyd A. Horrocks

Book ID
103150106
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
957 KB
Volume
673
Category
Article
ISSN
1873-3778

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✍ Chi-Sun Wang πŸ“‚ Article πŸ“… 1980 πŸ› Elsevier Science 🌐 English βš– 611 KB

Sequential chromatography of human milk whey on concanavalin A-Sepharose 4B followed by cholate-Sepharose 4B yielded a bile salt-activated lipase with M-fold purification. The lipase was not retained by concanavalin A-Sepharose 4B but was retained by the cholate-Sepharose 4B, from which it was elute