Purification of Interferon γ-Interferon γ Receptor Complexes by Preparative Electrophoresis on Native Gels

✍ Scribed by M. Fountoulakis; E. Takacsdilorenzo; J.F. Juranville; M. Manneberg

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 829 KB
• Volume: 208
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1045

✦ Synopsis

Cytokine-receptor complexes are required for certain studies including crystallization, NMR spectra, and investigation of the biological response mechanism to the cytokine. The purity of the ligand-receptor complex is critical for most of these applications. We investigated the possibility of purifying protein-protein complexes by electrophoresis on native gels. Starting with partially purified mouse and highly purified human proteins, we prepared milligram amounts of interferon (\gamma)-interferon (\gamma) receptor complexes by preparative electrophoresis on nondenaturing polyacrylamide gels. In both cases, pure ligand-receptor complexes with the correct stoichiometry of binding were recovered. Electrophoresis on preparative native gels may prove to be of general interest for the preparation of protein-protein complexes to be used in diverse studies. (\mathbb{C} 1993) Academic Press, Inc.