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Purification of influenza virus hemagglutinin by affinity chromatography

✍ Scribed by H. Becht; R. Rott


Publisher
Springer-Verlag
Year
1972
Tongue
English
Weight
230 KB
Volume
158
Category
Article
ISSN
0300-8584

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Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B-antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column