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Purification of hydrophobic integral membrane proteins from Mycoplasma hyopneumoniae by reversed-phase high-performance liquid chromatography

โœ Scribed by R.P. Lee; S.W. Doughty; K. Ashman; J. Walker


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
521 KB
Volume
737
Category
Article
ISSN
1873-3778

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Reverse-phase high-performance liquid chromatography (HPLC) resolution and recovery of cytochrome P-450 and bovine rhodopsin, both integral membrane proteins, and large peptides derived from P-450 LMI were enhanced by utilizing ternary solvents. Surprisingly, most test materials eluted later in the