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Purification of human serum hemopexin by chromatography on DEAE-cellulose

✍ Scribed by Z. Hrkal; Z. Vodrážka; J. Rejnková


Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
281 KB
Volume
72
Category
Article
ISSN
1873-3778

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A relatively simple procedure is described for purifying human serum angiotensin-converting enzyme. The enzyme was purified 130,000-fold to electrophoretic homogeneity using affinity chromatography as the principal purification step. The ligand was an immobilized competitive inhibitor, D-cysteinyl-L