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Purification of human haptoglobin 1-1, 2-1, and 2-2 using monoclonal antibody affinity chromatography

✍ Scribed by Chi Feng Tseng; Hsing Yi Huang; Yuan Ting Yang; Simon J.T Mao

Book ID
116827740
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
281 KB
Volume
33
Category
Article
ISSN
1046-5928

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An affinity purification technique has been developed using mild elution conditions for the isolation of humanized murine and murine \(\mathrm{IgG}_{1}\). This technique is based on the innate affinity of \(\mathrm{IgG}_{1}\) for metal and utilizes immobilized metal-affinity chromatography. Antibody