Purification of heparin, dermatan sulfate and chondroitin sulfate from mixtures by sequential precipitation with various organic solvents

Fractionation of Heparin, Dermatan Sulfa

Fractionation of Heparin, Dermatan Sulfate, and Chondroitin Sulfate by Sequential Precipitation: A Method to Purify a Single Glycosaminoglycan Species from a Mixture

✍ N. Volpi 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 882 KB

Purified heparin, dermatan sulfate, and chondroitin sulfate in mixtures were fractionated by sequential precipitation with increasing volumes of acetone and analyzed by agarose-gel electrophoresis and for M(r), charge density, constituent disaccharides, and anticoagulant activity (for heparin). Puri