Purification of glycogen debranching enzyme from rabbit muscle using ω-aminoalkyl agarose chromatography

✍ Scribed by R.C. White; C.J. Ruff; T.E. Nelson

• Book ID: 102627884
• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 253 KB
• Volume: 115
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90022-1

✦ Synopsis

Rabbit muscle glycogen debranching enzyme binds to all of a homologous series of waminoalkyl agaroses. The debrancher can be eluted from w-aminoethyl and w-aminobutyl agarose with 0.5 M NaCI, and it desorbs more readily and elutes sooner from w-aminoethyl agarose than from w-aminobutyl agarose. No activity is eluted from w-aminohexyl, octyl, or decyl agaroses. An improved purification procedure has been developed which includes chromatography on w-aminoethyl agarose. This procedure enables the isolation of over 90% yield of the debranching enzyme from muscle within 3 days.