Purification of fusion proteins using affinity microspheres in aqueous two-phase systems
β Scribed by Akihiko Kondo, Tetsuya Kaneko, Ko Higashitani
- Book ID
- 120768633
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 755 KB
- Volume
- 40
- Category
- Article
- ISSN
- 1432-0614
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π SIMILAR VOLUMES
Aqueous polymer three-phase systems composed of dextran-Ficoll-polyethylene glycol-water have been used for affinity partition of proteins. The upper, middle, and lower phases are rich in polyethylene glycol, Ficoll, and dextran, respectively. Affinity partition was performed using the reactive dyes
Aqueous two-phase systems containing two polymers are a mild separation method for biomolecules due to the high concentration of water (75-90% ) in the phases. The phase systems have been further developed by introduction of thermoseparating polymers which make recycling of phase components possible