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Purification of exopolygalacturonase by affinity chromatography on Concanavalin A — Bead cellulose

✍ Scribed by E. Stratilová; D. Mislovičová; M. Dzúrová

Book ID
104651308
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
254 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis

Interaction of exopolygalacturonase isolated from carrot roots with Concanavalin A was investigated. The glycoprotein character of this enzyme was confirmed. Using affinity chromatography on Concanavalin A -triazine bead cellulose about fifty fold purification was achieved. The individual enzyme forms were not separated by this method, which might imply identical or similar pattern of N-glycosylation.

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