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Purification of ethanolaminephosphotransferase from bovine liver microsomes

โœ Scribed by Alessandra Mancini; Francesca Del Rosso; Rita Roberti; Pierluigi Orvietani; Lucia Coletti; Luciano Binaglia


Book ID
117772485
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
373 KB
Volume
1437
Category
Article
ISSN
1388-1981

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โœ John R. Wettereau; Donald B. Zilversmit ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 921 KB

A lipid transfer protein was isolated from bovine liver. Following the release of soluble proteins from liver microsomes, the transfer protein was purified 75-fold to near homogeneity by a combination of DEAE-celiulose ion exchange, Sephadex G-200 gel permeation, and hydroxylapatite chromatography.