Purification of endogenous inhibitors of [3H]flunitrazepam binding from bovine brain
โ Scribed by Hiroaki Kawasaki; Masatoshi Itoh; Tatsuo Nakahara; Akito Nohtomi; Motofumi Fukahori
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 698 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0364-3190
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๐ SIMILAR VOLUMES
A calcium-activated neutral proteinase (CANP)-specific endogenous inhibitor (calpastatin) was purified from bovine brain b y successive column chromatography. The purified inhibitor exhibited a major band on sodium dodecylsulfate polyacrylamide gel electrophoresis with an approximate molecular weigh
One of the endogenous substances which modulate glutamate receptor binding was isolated and highly purified from porcine brain. The purification involved extraction of brain tissue with doubled distilled water, followed by gel filtration, anion exchange, cation exchange, and several steps of C18 rev