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Purification of cytochrome P-450 from pig testis by aniline-sepharose 4B and isoelectric focusing

✍ Scribed by Masahiro Kuwada; Yuko Furuse; Hideo Hasumi; Jun Maki

Book ID
111743023
Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
677 KB
Volume
47
Category
Article
ISSN
1521-6543

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πŸ“œ SIMILAR VOLUMES

A two-step purification of cytochrome P-
A two-step purification of cytochrome P-450 from adult pig testis by pregnenolone affinity column chromatography
✍ Masahiro Kuwada πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 141 KB

Adult testicular cytochrome P-450 was purified by a two-step procedure utilizing hydroxylapatite and pregnenolone affinity column chromatography. The cytochrome P-450 was determined to have an isoelectric point of 6.43 on analytical isoelectric focusing. The purified cytochrome P-450 was found to be