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Purification of CpG islands using a methylated DNA binding column

โœ Scribed by Cross, Sally H.; Charlton, Jillian A.; Nan, Xinsheng; Bird, Adrian P.


Book ID
109915384
Publisher
Nature Publishing Group
Year
1994
Tongue
English
Weight
969 KB
Volume
6
Category
Article
ISSN
1061-4036

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The large-scale purification of plasmid DNA was achieved using fast protein liquid chromatography on a Hi-Load Q Sepharose column. This method allows for the purification of plasmids starting from crude plasmid DNA, prepared by a simple alkaline lysis procedure, to pure DNA in less than 5 h. In cont