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Purification of component A of the soluble methane monooxygenase of Methylococcus capsulatus (Bath) by high-pressure gel permeation chromatography

✍ Scribed by Marc P. Woodland; Howard Dalton

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 510 KB
Volume: 139
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90034-4

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✦ Synopsis

A major improvement in the purification of the oxygenase protein (component A) of the methane monooxygenase has been effected. By employing high-pressure gel permeation chromatography several purification steps may be omitted from the previously published scheme. Furthermore the yield of the protein is enhanced and more importantly the recovered protein displays an increased specific activity, unlike that purified by other techniques.

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