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Purification of circular YACs from yeast cells for DNA sequencing

✍ Scribed by Belzile, Francois; Leem, S.-H.; Yoon, Y.-H.; Kim, S. I.; Larionov, V.

Book ID
121251682
Publisher
NRC Research Press
Year
2008
Tongue
English
Weight
125 KB
Volume
51
Category
Article
ISSN
0831-2796

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A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif