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Purification of catechol-O-methyltransferase by affinity chromatography

✍ Scribed by Ronald T. Borchardt; Chao-Fu Cheng; Dhiren R. Thakker

Book ID
119405745
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
520 KB
Volume
63
Category
Article
ISSN
0006-291X

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Purification of rat liver soluble catech
Purification of rat liver soluble catechol-O-methyltransferase by high performance liquid chromatography
✍ Tapio Korkolainen; Erkki Nissinen πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 324 KB πŸ‘ 2 views

The soluble form of catechol-0-methyltransferase (EC 2.1.1.6.) from rat liver was purified to homogeneity by high-performance anion-exchange chromatography and high-performance gel-filtration chromatography. The specific activity of the final pool was 270 U/mg protein. The purification was 1180-fold