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Purification of Carboxypeptidase B by Zinc Chelate Chromatography

✍ Scribed by Hortin, G.L.; Gibson, B.L.

Book ID
121513727
Publisher
Informa UK (Taylor & Francis)
Year
1989
Tongue
English
Weight
390 KB
Volume
19
Category
Article
ISSN
0032-7484

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Copper, zinc superoxide dismutase was isolated from human red blood cell hemolysate by DEAE-Sepharose and copper chelate affinity chromatography. Enzyme preparations had specific activities ranging from 3400 to 3800 U/mg and recoveries were approximately 60% of the enzyme activity in the lysate. Cop