𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Purification of Brain Tubulin-Tyrosine Ligase by Biochemical and Immunological Methods

✍ Scribed by Heinz C. Schröder, Jürgen Wehland and Klaus Weber

Book ID
124787690
Publisher
Rockefeller University Press.
Year
1985
Tongue
English
Weight
473 KB
Volume
100
Category
Article
ISSN
0021-9525

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Release of [14C]tyrosine from tubulinyl-[14C]tyrosine by brain extract. Separation of a carboxypeptidase from tubulin-tyrosine ligase
✍ Carlos E. Argaraña; Hector S. Barra; Ranwel Caputto 📂 Article 📅 1978 🏛 Springer 🌐 English ⚖ 343 KB

The carboxypeptidase previously described that releases tyrosine from tubulinyl-tyrosine was obtained from rat brain preparation free of tubulin-tyrosine ligase. The enzyme was purified 24-fold. Its activity was increased by 2 mM MgCl2 or 30 mM KCl. Mercaptoethanol (50 mM), colchicine (0.2 mM) and t