Purification of a soluble monoisozyme of nucleoside diphosphate kinase from chick brain: Exploitation of ionic characteristics

✍ Scribed by Khalid Islam; Roy G. Burns

• Book ID: 102986114
• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 517 KB
• Volume: 137
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90338-5

✦ Synopsis

A procedure for the rapid purification of nucleoside diphosphate kinase, 24 h with a single operator, from the chick brain soluble fraction is described. The influence of the ionic conditions on the association-disassociation properties of the enzyme are exploited to obtain yields of 30% from the crude homogenate. The enzyme has been purified 500-fold with a maximal specific activity of 1500 mumol/min/mg at 25 degrees C (using thymidine diphosphate as the phosphate acceptor and ATP as the donor) and is demonstrated to be monoisozymic.