Purification of a soluble cytochrome P450 from Trichosporon montevideense
✍ Scribed by Ute M. Stündl; Dirk Patzak; Frieder Schauer
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 65 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0233-111X
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NADPH-cytochrome P-450 oxidoreductase (P-450 red) transfers reducing equivalents from NADPH to cytochrome P-450 (P-450) in the monooxygenase system. Detergent solubilized proteins from the membrane fraction of neonatal rat epidermis were purified by 2',5'-ADP-agarose affinity column chromatography.
## Abstract A new form of cytochrome P‐450 was partially purified from hepatic microsomes of neonatally imprinted rats (adult male and adult male castrated at four weeks of age). This new form of cytochrome P‐450 appears to have an apparent molecular weight of approximately 50,000 daltons as judged