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Purification of a protein kinase from human Namalwa cells that phosphorylates topoisomerase I

โœ Scribed by John S. Mills; Harris Busch; Egon Durban


Book ID
115756158
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
757 KB
Volume
109
Category
Article
ISSN
0006-291X

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Purification of p53/55 kinase from nucle
โœ Derek A. Graham; Jahar K. Deb; Harris Busch ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 901 KB

A nuclear p53/55 protein kinase has been isolated from nuclear ribonucleoprotein particles from human tumor cells. The enzyme was purified approximately 2200-fold cell nuclei by sequential ribonuclease digestion of the RNP particles, DEAE cellulose and phosphocellulose chromatography. The kinase whi