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Purification of a phosphate carrier in pig heart mitochondria by affinity chromatography on mersalyl—ultrogel

✍ Scribed by Sylvie Touraille; Yves Briand; Rogert Durand; Jean-Claude Bonnafous; Jean-Claude Mani

Book ID
115912793
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
337 KB
Volume
128
Category
Article
ISSN
0014-5793

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Rapid large-scale purification of pig he
Rapid large-scale purification of pig heart nucleoside diphosphate kinase by affinity chromatography on Cibacron Blue 3G-A Sepharose
✍ I. Lascu; M. Duc; A. Cristea 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 436 KB

A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography