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Purification of a constitutive chitosanase produced by Bacillus sp. MET 1299 with cloning and expression of the gene

✍ Scribed by Pyoung Il Kim; Tae Heung Kang; Kyoung Jin Chung; In Seon Kim; Ki-chul Chung


Book ID
108131130
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
689 KB
Volume
240
Category
Article
ISSN
0378-1097

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✍ Young-Jung Wee; Lebaka Veeranjaneya Reddy; Soon-Do Yoon; Hwa-Won Ryu 📂 Article 📅 2011 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 133 KB

BACKGROUND: Purification and enzymatic properties of a chitosanase from Bacillus subtilis RKY3 have been investigated to produce a chitooligosaccharide. The enzyme reported was extracellular and constitutive, which was purified by two sequential steps including ammonium sulfate precipitation and ion