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Purification of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans : An iron-sulfur protein

✍ Scribed by Georg SCHWEIGER; Roland DUTSCHO; Wolfgang BUCKEL


Book ID
115125447
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
790 KB
Volume
169
Category
Article
ISSN
1432-1327

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Anaerobically prepared cell-free extracts from Fusobacterium nucleatum contain 2-hydroxyglutaryl-CoA dehydratase with a specific activity of 20 nkat mg-1. The enzyme was purified 24-fold to a specific activity of 480 nkat mg-1 by anion exchange chromatography, gel filtration and chromatography on Bl