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Purification in high yield and characterisation of the galactose-specific lectin from the seeds of snake gourd (Trichosanthes anguina)

✍ Scribed by Sneha Sudha Komath; Siva Kumar Nadimpalli; Musti J. Swamy

Book ID
111742033
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
612 KB
Volume
39
Category
Article
ISSN
1521-6543

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Fluorescence quenching, time-resolved fl
Fluorescence quenching, time-resolved fluorescence and chemical modification studies on the tryptophan residues of snake gourd (Trichosanthes anguina) seed lectin
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Fluorescence quenching and time-resolved fluorescence studies have been performed on the galactose-specific lectin purified from snake gourd (Trichosanthes anguina) seeds, in order to investigate the tryptophan accessibility and environment in the native protein and in the presence of bound ligand.

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## Abstract A galactose‐specific lectin has been purified from the seeds of __Trichosanthes cordata__ by affinity chromatography on crosslinked guar gum. The affinity‐eluted lectin could be resolved into two isolectins, TCA‐I and TCA‐II by ion‐exchange chromatography on DEAE cellulose. The molecula