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Purification by reverse-phase high-performance liquid chromatography of an epidermal growth factor-dependent transforming growth factor

✍ Scribed by Mario A. Anzano; Anita B. Roberts; Joseph M. Smith; Lois C. Lamb; Michael B. Sporn

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
897 KB
Volume
125
Category
Article
ISSN
0003-2697

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✦ Synopsis

Methods

Chemicals. Bio-Gel P-30 ( 100-200 mesh), protein assay kit, and reagents used for polyacrylamide gel electrophoresis were purchased from Bio-Rad Laboratories (Richmond, Calif.). Low molecular weight protein standards for gel electrophoresis were obtained from Pharmacia (Piscataway, N. J.) and Bethesda Research Laboratories (Gaithersburg, Md.). Acetonitrile and npropanol for HPLC were products of Burdick and Jackson Laboratories (Muskegon, Mich.). Sequanal grade trifluoroacetic acid (TFA) was obtained from Pierce Chemical Company (Rockford, Ill.). Deionized water was distilled in glass. Agar Noble was ob-217

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