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Purification and spectrophotometric assay of neomycin phosphotransferase II

✍ Scribed by Patricia R. Goldman; Dexter B. Northrop


Book ID
119406339
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
362 KB
Volume
69
Category
Article
ISSN
0006-291X

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Assay of neomycin phosphotransferase act
✍ N. Ramesh; William R.A. Osborne πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 293 KB

A simple method for the measurement of neomycin phosphotransferase (NPT) activity in crude extracts of eukaryotic cells is described. This method is based on the elimination of interfering phosphorylated proteins by using phenol-chloroform extraction. This solution phase assay allows the detection o