Assay of neomycin phosphotransferase act
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N. Ramesh; William R.A. Osborne
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Article
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1991
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Elsevier Science
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English
β 293 KB
A simple method for the measurement of neomycin phosphotransferase (NPT) activity in crude extracts of eukaryotic cells is described. This method is based on the elimination of interfering phosphorylated proteins by using phenol-chloroform extraction. This solution phase assay allows the detection o