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Purification and some regulatory properties of glycogen synthase I from rabbit renal medulla

✍ Scribed by Schlender, Keith K.; Doster, Christine M.


Book ID
122449521
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
630 KB
Volume
71
Category
Article
ISSN
1096-4959

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Glycogen synthase kinase-3 (GSK-3) was purified from rabbit liver to homogeneity by ultracentrifugation, ion-exchange chromatography on DEAE-cellulose, Cellulose phosphate, CM-Sephadex and Fast Protein Liquid Chromatography (FPLC) on Mono-S column. The enzyme was purified approximately 20,000 fold w