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Purification and Some Properties of Threonine Dehydratase from Yeast

✍ Scribed by Tsunehiko Katsunuma; Sigrid Elsässer; Helmut Holzer


Book ID
115112947
Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
527 KB
Volume
24
Category
Article
ISSN
1432-1327

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Purification and some properties of thre
✍ Doz. Dr. R. Bode; I. Schult; D. Birnbaum 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 618 KB

Threonine dehydratase has been purified about 200-fold from extracts of Candida maltosa by chromatography on DEAE-cellulose, Sephadex G-200, and hydroxylapatite. The enzyme is fairly stable in solution in the presence of 250 mM phosphate buffer containing 40% glycerol. The molecular weight of the na